跳至正文

Our Latest Publications

2025年

  • Zhang, Y., Zhang, T., Xiao, X., Wang, Y., Kawalek, A., Ou, J., Ren, A., Sun, W., de Bakker, V., Liu, Y., et al. Xue Liu (2025). CRISPRi screen identifies FprB as a synergistic target for gallium therapy in Pseudomonas aeruginosa. Nat Commun 16, 5870. https://doi.org/10.1038/s41467-025-61208-z.
  • Rengifo-Gonzalez, M., Mazzuoli, M.-V., Janssen, A.B., Rueff, A.-S., Burnier, J., Liu, X., and Veening, J.-W. (2025). Make-or-break prime editing for genome engineering in Streptococcus pneumoniae. Nat Commun 16, 3796. https://doi.org/10.1038/s41467-025-59068-8.

2024年

  • Liu, X., Van Maele, L., Matarazzo, L., Soulard, D., Alves Duarte da Silva, V., de Bakker, V., Dénéréaz, J., Bock, F.P., Taschner, M., Ou, J., et al. (2024). A conserved antigen induces respiratory Th17-mediated broad serotype protection against pneumococcal superinfection. Cell Host Microbe 32, 304-314.e8. https://doi.org/10.1016/j.chom.2024.02.002.
  • Liu, X., de Bakker, V., Heggenhougen, M.V., Mårli, M.T., Frøynes, A.H., Salehian, Z., Porcellato, D., Morales Angeles, D., Veening, J.-W., and Kjos, M. (2024). Genome-wide CRISPRi screens for high-throughput fitness quantification and identification of determinants for dalbavancin susceptibility in Staphylococcus aureus. mSystems 9, e01289-23. https://doi.org/10.1128/msystems.01289-23.
  • Jana, B., Liu, X., Dénéréaz, J., Park, H., Leshchiner, D., Liu, B., Gallay, C., Zhu, J., Veening, J.-W., and van Opijnen, T. (2024). CRISPRi-TnSeq maps genome-wide interactions between essential and non-essential genes in bacteria. Nat Microbiol 9, 2395–2409. https://doi.org/10.1038/s41564-024-01759-x.
  • Zhou, Y., Song, Y., Zhang, Y., Liu, X., Liu, L., Bao, Y., Wang, J., and Yang, L. (2024). Azalomycin F4a targets peptidoglycan synthesis of Gram-positive bacteria revealed by high-throughput CRISPRi-seq analysis. Microbiol Res 280, 127584. https://doi.org/10.1016/j.micres.2023.127584.
  • Barbuti, M.D., Lambert, E., Myrbråten, I.S., Ducret, A., Stamsås, G.A., Wilhelm, L., Liu, X., Salehian, Z., Veening, J.-W., Straume, D., et al. (2024). The function of CozE proteins is linked to lipoteichoic acid biosynthesis in Staphylococcus aureus. mBio 15, e0115724. https://doi.org/10.1128/mbio.01157-24.

2023年

  • Zhang, H., Li, X., Liu, X., Ji, X., Ma, X., Chen, J., Bao, Y., Zhang, Y., Xu, L., Yang, L., et al. (2023). The usnic acid derivative peziculone targets cell walls of Gram-positive bacteria revealed by high-throughput CRISPRi-seq analysis. Int J Antimicrob Agents 62, 106876. https://doi.org/10.1016/j.ijantimicag.2023.106876.
  • Sun, D., Liu, X., and Yang, X. (2023). Editorial: The spatial-temporal dynamics of host-pathogen interaction during inflammatory disease. Front Cell Infect Microbiol 13, 1308419. https://doi.org/10.3389/fcimb.2023.1308419.
  • Minhas, V., Domenech, A., Synefiaridou, D., Straume, D., Brendel, M., Cebrero, G., Liu, X., Costa, C., Baldry, M., Sirard, J.-C., et al. (2023). Competence remodels the pneumococcal cell wall exposing key surface virulence factors that mediate increased host adherence. PLoS Biol 21, e3001990. https://doi.org/10.1371/journal.pbio.3001990.

2022年

  • Dewachter, L., Dénéréaz, J., Liu, X., de Bakker, V., Costa, C., Baldry, M., Sirard, J.-C., and Veening, J.-W. (2022). Amoxicillin-resistant Streptococcus pneumoniae can be resensitized by targeting the mevalonate pathway as indicated by sCRilecs-seq. Elife 11, e75607. https://doi.org/10.7554/eLife.75607.
  • Huang, W., Zhang, J., He, Y., Hu, C., Cheng, S., Zeng, H., Zheng, M., Yu, H., Liu, X., Zou, Q., et al. (2022). A cyclic adenosine monophosphate response element-binding protein inhibitor enhances the antibacterial activity of polymyxin B by inhibiting the ATP hydrolyzation activity of CrrB. Front Pharmacol 13, 949869. https://doi.org/10.3389/fphar.2022.949869.
  • Dong, N., Zeng, Y., Wang, Y, Liu, C., Lu, J., Cai, C., Liu, X., Chen, Y., Wu, Y., Fang, Y., et al. (2022). Distribution and spread of the mobilised RND efflux pump gene cluster tmexCD-toprJ in clinical Gram-negative bacteria: a molecular epidemiological study. Lancet Microbe 3, e846–e856. https://doi.org/10.1016/S2666-5247(22)00221-X.

2021年

  • Liu, X., Kimmey, J.M., Matarazzo, L., de Bakker, V., Van Maele, L., Sirard, J.-C., Nizet, V., and Veening, J.-W. (2021). Exploration of Bacterial Bottlenecks and Streptococcus pneumoniae Pathogenesis by CRISPRi-Seq. Cell Host & Microbe 29, 107-120.e6. https://doi.org/10.1016/j.chom.2020.10.001.
  • de Bakker, V., Liu, X., Bravo, A.M., and Veening, J.-W. (2022). CRISPRi-seq for genome-wide fitness quantification in bacteria. Nat Protoc 17, 252–281. https://doi.org/10.1038/s41596-021-00639-6.

2020年及以前

  • Liu, X., Li, J.-W., Feng, Z., Luo, Y., Veening, J.-W., and Zhang, J.-R. (2017). Transcriptional Repressor PtvR Regulates Phenotypic Tolerance to Vancomycin in Streptococcus pneumoniae. J Bacteriol 199. https://doi.org/10.1128/JB.00054-17.
  • Liu, X., Gallay, C., Kjos, M., Domenech, A., Slager, J., Kessel, S.P. van, Knoops, K., Sorg, R.A., Zhang, J.-R., and Veening, J.-W. (2017). High‐throughput CRISPRi phenotyping identifies new essential genes in Streptococcus pneumoniae. Molecular Systems Biology 13, 931. https://doi.org/10.15252/msb.20167449（cover paper）.
  • Zhang, B., Liu, X., Lambert, E., Mas, G., Hiller, S., Veening, J.-W., and Perez, C. (2020). Structure of a proton-dependent lipid transporter involved in lipoteichoic acids biosynthesis. Nat Struct Mol Biol 27, 561–569. https://doi.org/10.1038/s41594-020-0425-5.
  • Wang, X., Zeng, Y., Sheng, L., Larson, P., Liu, X., Zou, X., Wang, S., Guo, K., Ma, C., Zhang, G., et al. (2019). A Cinchona Alkaloid Antibiotic That Appears To Target ATP Synthase in Streptococcus pneumoniae. J. Med. Chem. 62, 2305–2332. https://doi.org/10.1021/acs.jmedchem.8b01353.
  • Zhu, D., Wang, L., Shang, G., Liu, X., Zhu, J., Lu, D., Wang, L., Kan, B., Zhang, J.-R., and Xiang, Y. (2014). Structural biochemistry of a Vibrio cholerae dinucleotide cyclase reveals cyclase activity regulation by folates. Mol Cell 55, 931–937. https://doi.org/10.1016/j.molcel.2014.08.001.
  • Yang, H., Liu, X., Li, Q., Li, L., Zhang, J.-R., and Tang, Y. (2016). Total synthesis and preliminary SAR study of (±)-merochlorins A and B. Org Biomol Chem 14, 198–205. https://doi.org/10.1039/c5ob01946j.
  • He, L., Nair, M.K.M., Chen, Y., Liu, X., Zhang, M., Hazlett, K.R.O., Deng, H., and Zhang, J.-R. (2016). The Protease Locus of Francisella tularensis LVS Is Required for Stress Tolerance and Infection in the Mammalian Host. Infect Immun 84, 1387–1402. https://doi.org/10.1128/IAI.00076-16.
  • Wen, Z., Sertil, O., Cheng, Y., Zhang, S., Liu, X., Wang, W.-C., and Zhang, J.-R. (2015). Sequence elements upstream of the core promoter are necessary for full transcription of the capsule gene operon in Streptococcus pneumoniae strain D39. Infect Immun 83, 1957–1972. https://doi.org/10.1128/IAI.02944-14.
  • Song, Y, Li, Q., Liu, X., Chen, Y., Zhang, Y., Sun, A., Zhang, W., Zhang, J., and Ju, J. (2014). Cyclic Hexapeptides from the Deep South China Sea-Derived Streptomyces scopuliridis SCSIO ZJ46 Active Against Pathogenic Gram-Positive Bacteria. J Nat Prod 77, 1937–1941. https://doi.org/10.1021/np500399v.